airway cells - viral infection
Respiratory Assays
Airway Cells - Viral Infection
Cytokine release after Respiratory Syncytial Virus (RSV) infection
Time-course of A549 cell viability and cytotoxicity (LDH release) following hRSV infection.
A549 cells were infected with hRSV (3 MOIs). Viability was measured by CellTiterGlo® assay at days 1, 2, 3, and 9 vs uninfected control, in technical triplicates. % LDH release was measured by LDH-GLO® assay at days 3, 5, and 7 post infection relative to Triton X-100 positive control, in technical triplicates.
Cytokine release after RSV infection and Dexamethasone treatment
Dexamethasone modulates hRSV-induced inflammatory mediator release in A549 cells.
A549 cells infected with hRSV ± dexamethasone; panel of cytokines quantified in culture supernatants by Luminex Assay (n=3). (Stats: one-way ANOVA; n as stated; mean ± SEM; *p<0.05, **p<0.01, ***p<0.001)
Cytokine release after RSV infection and Presatovir treatment
Presatovir modulates hRSV-induced inflammatory responses in A549 cells.
A549 cells were infected with hRSV in the presence or absence of Presatovir; a panel of cytokines/chemokines was quantified in culture supernatants using the Luminex Assay at 24 and 72 hours post-infection (n = 3). (Stats: one-way ANOVA; n as stated; mean ± SEM; *p<0.05, **p<0.01, ***p<0.001)
Cytokine release after RSV infection and Ribavirin treatment
Ribavirin modulates hRSV-induced inflammatory mediator release in A549 cells.
A549 cells were infected with hRSV ± Ribavirin; a panel of cytokines and chemokines was quantified in culture supernatants using the Luminex Assay at 24 and 72 hours post-infection (n = 3). (Stats: one-way ANOVA; n as stated; mean ± SEM; *p<0.05, **p<0.01, ***p<0.001).
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