bronchial epithelial stress responses

Respiratory Assays

Bronchial Epithelial Stress Responses

The models of bronchial epithelial stress responses help researchers investigate how airway epithelial cells react to environmental, inflammatory, and oxidative challenges. These advanced in vitro systems enable the assessment of cellular signalling pathways, barrier integrity, cytokine release, and stress-induced molecular changes that contribute to respiratory disease progression.

HIF-mediated signalling, and matrix remodelling under hypoxic airway conditions

HIF1α expression increases in ALI bronchial epithelial cultures under hypoxic-mimetic stress.

Primary human bronchial epithelial cells (HBEpC) from healthy and COPD donors in 14-day ALI cultures treated with CoCl₂ or BiPS; HIF1α levels measured and normalized to total protein (BCA assay) (Stats: one-way ANOVA; n as stated; mean ± SEM; *p<0.05, **p<0.01, ***p<0.001)

Hypoxia or stabilizator–induced COPD markers in Healthy and COPD cells

Stress responses: Hypoxia- and TGF-β–driven fibrotic activation and remodelling

Hypoxia- and TGF-β–associated fibrotic gene expression in normal (HPF) vs IPF fibroblasts.

HPF and IPF fibroblasts were treated for 6 hours with CoCl₂, TGF-β1, or SB431542 (with TGF-β1), and COL1A1, ACTA2, and VEGFA expression was quantified and normalized to GAPDH. (Stats: one-way ANOVA; n as stated; mean ± SEM; *p<0.05, **p<0.01, ***p<0.001).

Studies of bronchial epithelial stress responses provide valuable insights into disease mechanisms associated with chronic respiratory disorders and airway dysfunction.

Request a consultation with Cellomatics Biosciences today

Cellomatics offers customised assays focused on bronchial epithelial stress responses to support respiratory drug discovery and translational research programmes. Our experienced team of in vitro laboratory scientists will work with you to understand your project and provide a bespoke project plan with a professional, flexible service and a fast turnaround time.

To request a consultation where we can discuss your exact requirements, please contact Cellomatics Biosciences.