Oncology Assays
DNA Damage
DNA carries the instructions essential for cellular function, and within a single cell it stretches to around two metres in length, making it highly susceptible to damage. The DNA within a cell nucleus is exposed to numerous factors which can induce damage (e.g., environmental carcinogens, genotoxic compounds, oxidizing agents such as hydrogen peroxide); damage also builds up naturally through the day. To maintain genomic stability, cells rely on multiple repair pathways that detect and correct damage, often in the form of single- or double-strand breaks, and measuring these events provides insight into DNA integrity.
Get in touch to find out more about the migration assays we offer at Cellomatics.
COMET Assays
The COMET assay (single cell gel electrophoresis) can detect and quantify DNA strand breakages. Cells are embedded in agarose on a microscope slide and strands of damaged DNA migrate out of the cell when an electric current is applied. The distance the DNA migrates, and the overall shape, appears like a comet. The amount of migration corresponds to the amount of strand breakages; this can be quantified by a computer after the DNA has been labelled with a fluorescent marker.
Images show representative ROIs from triplicate TriTek COMET slides (10x objective). Cells were treated for 30 minutes in HBSS or HBSS+200μM H2O2. Development of COMET tails can be seen following exposure to the DNA damaging agent. 100 COMETs per treatment was measured and analysed using COMETScore 2.0 software. T-test demonstrates peroxide significantly induces DNA damage (±SEM).
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Our experienced team of in vitro laboratory scientists will work with you to understand your project and provide a bespoke project plan with a professional, flexible service and a fast turnaround time.
To request a consultation where we can discuss your exact requirements, please contact Cellomatics Biosciences.
