Antibody-dependent cellular cytotoxicity (ADCC), also referred to as antibody-dependent cell-mediated cytotoxicity, is a mechanism of cell-mediated immune defence whereby an effector cell of the immune system actively lyses a target cell, whose membrane-surface antigens have been bound by specific antibodies. It is one of the mechanisms through which antibodies, as part of the humoral immune response, can act to limit and contain infection.

Antibody-dependent cellular cytotoxicity (ADCC)

Raji cells were treated with Anti-CD20 antibody and T cells and the Relative Luminescence Units (RLU) were measured 6 hours [A] and 24 hours [B] post incubation. The higher concentration of the Anti-CD20 resulted in an increase in the activation of the NFAT pathway. This corresponds directly to an increase in the target cell death.

Assessment of ADCC via GFP release

Incubation of MCF7 target cells with both Trastuzumab and PMA activated Thp1 effector cells induces a loss of intracellular GFP.

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