Senescence is the process by which cells irreversibly stop dividing and enter a state of permanent growth arrest without undergoing cell death. Senescence can be induced by unrepaired DNA damage or other cellular stresses and is often lined with ageing, cancer and fibrosis.

Primary human chondrocytes were treated with H2O2 for 24 hours followed by 72 hours recovery period and then treatment with ABT 263. Cells were fixed followed by immunofluorescence staining for DAPI and β-galactosidase was performed.

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