Antibody-dependent cellular phagocytosis is the mechanism by which antibody-opsonized target cells activate the FcγRs on the surface of macrophages to induce phagocytosis, resulting in the internalization and degradation of the target cell through acidification of the phagosome.

Antibody-Dependent Cellular Phagocytosis (ADCP)

The target Raji cells were pre-treated with or without Rituximab at different concentrations and stained with pHrodo Red (red). The THP-1 derived macrophages were stained with calcein (green). To assess antibody-dependent cellular phagocytosis of target Raji cells by THP-1 derived macrophages the two cell types were co-cultured for 6 hours. Data is represented as the red fluorescence intensity (RFI) at 0 hours vs. 6 hours in all treatment groups.

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