Immune Cell Killing

Natural killer cells and cytotoxic T lymphocytes (CTLs) perform complementary roles in immune responses directed against viruses and tumours. CTLs are antigen specific and recognize peptides derived from virus and tumour antigens presented by major histocompatibility complex (MHC) class I molecules. NK cells can recognize and kill cells that have down-regulated MHC class I molecules from their cell surface. The major cytotoxic proteins contained within secretory lysosomes in NK cells and CTLs are the granzymes and perforin. Target cell recognition induces secretory lysosome exocytosis and the release of the cytotoxic contents of this organelle. Perforin then facilitates the entry of the granzymes into the target cell cytoplasm, where they cleave a variety of targets, such as caspases, resulting in cell death (Topham and Hewitt, 2009).

Our scientific team can test the efficacy of your compounds/biologics in relation to their ability to potentiate/facilitate tumour cell killing by immune cells including T cells, NK cells, PBMCs

Immune cell killing

Quantification of Caspase 3/7 activity in a co-culture model of K562 cells incubated with unstimulated/stimulated primary CD56+ NK cells (**p<0.01; ***p<0.001; n=3 ± SEM). Staurosporine was used as a positive control.

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Our experienced team of in vitro laboratory scientists will work with you to understand your project and provide a bespoke project plan with a professional, flexible service and a fast turnaround time.

To request a consultation where we can discuss your exact requirements, please contact Cellomatics Biosciences.