Molecular Assays

Molecular assays explore the changes in a cell/tissue at the gene level using sophisticated techniques.

Gene Expression

Gene expression analysis is performed to identify the way genes are transcribed to synthesise functional gene products — functional RNA species or protein products. The study of gene regulation provides insights into normal cellular processes, such as differentiation, and abnormal or pathological processes.

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Quantigene

Multi-Plex Gene Assay – Unique Benefits
  • Sample types – Whole blood, FFPE, cells, serum, tissue lysates, no RNA purification required
  • Specific Probes – Each gene is specifically targeted by 6 different probes designed along the target
  • True amplification – The assay is based on branched DNA technology (sequential nucleic acid)
  • High precision – Coefficient of variation (CV) of less than 20% for entire process (sample isolation to results)
  • Multiplexing of targets – Up to 50 targets per well, housekeeping included
  • Multiple target species – Large inventory of validated genes for various species

Multi-Plex assay (xTAG)

Multi-plex Gene assay- Tissue

MSingle-Plex Gene Assay – Unique Benefits
  • Sample types – Whole blood, FFPE, cells, serum, tissue lysates, no RNA purification required
  • True amplification – The assay is based on branched DNA technology (sequential nucleic acid hybridization)
  • Easy workflow – Minimum sample preparation and ELISA like workflow
  • High precision – Coefficient of variation (CV) of less than 10% for entire process (sample isolation to results)

Single-Plex assay

Single-Plex Gene assay- In vitro samples

qRT-PCR (quantitative Real-time PCR)

  • Gold standard method to quantify gene expression, genotyping and melting curves
  • Up to 4-plex gene analysis in state-of-the-art equipment (QuantStudio™ 3)
  • Applicable to a wide range of biological samples, including very low starting material
  • Supports fast reactions (<40 mins) in a high-throughput manner (96-well plate format)
  • High specificity, sensitivity and reproducibility, particularly with predesigned TaqMan® assays
  • Perfectly streamlined with commercially available RNA extraction and cDNA synthesis kits

Amplification plot of COVID19 PCR detection kit using 3-plex qRT-PCR reaction in QuantStudio3. Amplification of target sequences ORF1ab and N gene is detected through the FAM and ROX channels, respectively, whereas the internal control (IC) is quantified in the VIC channel.

Request a consultation with Cellomatics Biosciences today

Our experienced team of in vitro laboratory scientists will work with you to understand your molecular assay requirements and provide a bespoke project plan with a professional, flexible service and a fast turnaround time.

To request a consultation where we can discuss your exact requirements, please contact Cellomatics Biosciences.