Collagen Gel Contraction
Contract gel contraction assay is used to study the cell-mediated reorganisation of the extracellular matrix. Hyperreactivity of smooth muscle cells/fibroblasts can be investigated using the collagen gel contraction principle.
Percentage change in gel contraction upon pre-treatment with 2,3 BDM followed by incubation for 4 hours with Carbachol. For each treatment the percentage change in the gel contraction was calculated by normalising to time 0. Each condition is representative of 3 biological replicates (n=3); mean+SEM. Data analysis performed using the 2-WAY ANOVA analysis, *p<0.05; **p<0.01, ***p<0.0001; Carbachol vs. test ingredients groups.
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