atopic dermatitis
Inflammation Assays
Atopic Dermatitis
Atopic dermatitis (AD) is a chronic, complex allergic inflammatory skin disease that affects 10-20% of the population, characterised by a periodic flare of the symptoms which include red and itchy skin, swollen and cracked. Initial immune response begins with the T helper (Th)2 response which then shifts to Th1 in the chronic phase.
Our team have the expertise to offer multiple cell-based approaches including:
- Primary Keratinocyte monoculture
- Human immortalized keratinocytes monoculture
- Primary/immortalised keratinocytes cocultured with immune cells
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Atopic dermatitis in vitro model
- Involves stimulation of human epidermal keratinocytes (NHEK) using a cocktail of proinflammatory agents, including Poly(I:C) and (TNF)-a (Th1cytokine) Or Poly(I:C), IL-4 and IL-13 (Th2 cytokines) to induce AD disease phenotype
- Measure expression levels of Eotaxin, IL1α, IL4, TNFα, GM-CSF, IL1β, IL8, TSLP, IL18, IFNα2, IFNβ1, MIP1α, RANTES, MIP3α, MDC, CCL27, involucrin and filaggrin to test the efficacy of your test compounds
- Explore TLR3/RLRs signaling: NFkB, IRF3 translocation/ phosphorylation; JAK/STAT, PI3K signalling to understand mechanism of action (MOA)Â
Atopic Dermatitis cell-based model
Primary healthy human keratinocytes were treated with different concentrations of JAK inhibitor along with Poly I:C+ recombinant IL-13 for 48 hours. Inflammatory markers in the resulting supernatants were analysed using a Human Magnetic Luminex® Assay (n=3, mean±SEM)
Atopic dermatitis – Frequently asked questions
Atopic dermatitis is a complex inflammatory skin disease involving skin epithelial barrier dysfunction, immune dysregulation, and chronic inflammation. To generate translationally relevant data, the challenge for any in vitro models is to capture both epithelial and immune-mediated responses associated with the disease.
Human skin models allow researchers to investigate inflammatory signalling, barrier integrity, and therapeutic responses in systems that more closely reflect human disease biology. These models can provide more physiologically relevant insights compared to conventional
approaches.
Atopic dermatitis is strongly associated with Th2-driven inflammation, particularly involving IL-4, IL-5 and IL-13, and TSLP signalling pathways. These pathways contribute to chronic inflammation, immune activation, and disruption of skin barrier homeostasi
In vitro atopic dermatitis models commonly include differentiated primary human keratinocytes grown on transwell systems, either as monocultures or in co-culture with primary human immune cells. These models can be established using cells derived from healthy donors or patients.
They provide reproducible and physiologically relevant platforms to investigate inflammatory signalling and skin barrier dysfunction, and are widely used for target validation, compound screening, and mechanistic studies during therapeutic development.
Common endpoints include cytokine release, gene expression profiling, skin barrier protein analysis, and assessment of immune activation markers. Additional assays including TEER are often used to evaluate epithelial integrity.
Request a consultation with Cellomatics Biosciences today
Our experienced team of in vitro laboratory scientists will work with you to understand your project and provide a bespoke project plan with a professional, flexible service and a fast turnaround time.
To request a consultation where we can discuss your exact requirements, please contact Cellomatics Biosciences.
