Cell adhesion is involved in stimulating signals that regulate cell differentiation, cell cycle, cell migration, and cell survival. We offer a range of adhesion assays that can support a variety of projects. Cell adhesion has application in cancer metastasis study, and in the determination of the adhesion properties of normal and cancerous cells.
Cellomatics Biosciences offer adhesion assays that can be applied to a wide range of projects.
Leukocyte adhesion using HUVEC co-culture
Human umbilical vein endothelial cells (HUVEC) were stimulated with TNFα in the presence or absence of the reference compound IC87114 (PI3K inhibitor). Fluorescently labelled peripheral blood derived human leucocytes were then cultured with HUVEC to determine leucocyte adhesion. A statistically significant increase in leukocyte adhesion was observed following TNFα stimulation of HUVEC monolayers. This effect was significantly inhibited by IC87114 (PI3K inhibitor). (*p<0.05; **p<0.01; ***p<0.001±SEM).
Effect of R-0021 test compound on Leukocyte adhesion. Leukocytes were treated with Histamine and two concentrations (1 µM and 10 µM) of R-0021 test compound. There was significant increase in alpha-2, 3, 4, 5-integrin mediated Leukocyte adhesion with Histamine treatment when compared to untreated (empty boxes). Higher concentration of R-0021 (10 µM) showed significant increase in the adhesion of Leukocytes by Alpha 3 and Alpha 4-Integrin when compared to untreated. Data is represented from 1 experiment conducted in triplicates and analysed using Two-way ANOVA followed by post-hoc Dunnett’s multiple comparisons test (**p<0.01; ***p<0.001; ±SEM).
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