Flow Cytometry (FACS)
Flow cytometry is a laser-based technology used to analyse the characteristics of cells or particles. It is a widely used method for analysing the expression of cell surface and intracellular molecules, characterising and defining different cell types in a heterogeneous cell population, assessing the purity of isolated subpopulations, and analysing cell size and volume. It allows simultaneous multi-parameter analysis of single cells.
THP-1 characterisation after PMA-differentiation
THP-1 (a model for human monocytes cells) on stimulation with PMA, differentiate and show macrophage-like phenotypes. The PMA-differentiated THP-1 cells were stained with macrophage surface markers (CD11; CD40) and analysed using FACS. From Figures A and B, it was observed that the expression of CD11 and CD40 was higher in PMA-differentiated THP-1 cells (red) when compared to undifferentiated THP-1 (green) cells.
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