Immunoassays

Immunoassays are bioanalytical methods used in drug discovery that use the specificity of an antigen-antibody reaction to detect and quantify target molecules in biological samples.

For more information on how Cellomatics Biosciences can assist with your immunoassays, please contact us or request a consultation.

Luminex Multiplex

xMAP Technology has application in serological assays by easy detection of antibodies. A serology assay enables the detection of an antibody (target) with the use of a capture protein antigen attached to the surface of a microsphere. A detection antibody that incorporates a fluorescent label is used to quantify the amount of target antibody present. This assay is useful when serum antibodies need to be measured, such as for the response to a vaccine, allergic reactions, or autoantibody detection.

It combines the efficiency and sensitivity of traditional ELISAs with high through-put capacity, small sample volume, efficiency in terms of time and cost,high reproducibility and sensitivity and analyte quantification over a wide range of concentrations.

Applications:

  • Measurement of cytokine/chemokine/inflammatory mediator expression in a variety of biological specimens which can be applied for biomarker discovery and Target validation
  • Allergy testing
  • Single nucleotide polymorphisms (SNPs) testing
  • Diagnosis of infectious diseases
  • Application areas include inflammation, autoimmune diseases, asthma, obesity/diabetes, neurological disorders, cancer, cardiovascular disease, drug discovery and vaccine development

Biomarker qualification and validation:

  • Robust validated technology
  • Simultaneous detection and quantification of multiple analytes in a single sample from a variety of biological specimens
  • High sensitivity and reproducibility
  • High through-put capacity
  • Efficiency in terms of time and cost
  • Various species

Multiplex Assay (xMAP)

Picture: Methods- ThermoFisher Scientific & R&D Systems®; Results- Cellomatics BioSciences Ltd.

Multiplex Analysis-Serum

Serum samples from 9 healthy volunteers were analysed for GDF1, Adam TS13 and sVAM-1 using Luminex Multiplex assay. Standard curves and median fluorescent intensities (MFIs) are extracted from the Luminex MAGPix instrument. The Exponent software (Luminex) analyses the MFIs for each sample and concentrations are calculated from the standard curves. These concentrations are then graphed on GraphPad Prism.

NoPlexAssay PanelDescription
141-plexHuman Cytokine/Chemokine Panel IsCD40L, EGF, Eotaxin/CCL11, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GRO, IFN-α2, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IP-10, MCP-1, MCP-3, MDC (CCL22), MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TGF-α, TNF-α, TNF-β, VEGF
229-plexHuman Cytokine/Chemokine Panel IIEGF, G-CSF, GM-CSF, IFN-α2, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IP-10, MCP-1, MIP-1α, MIP-1β, TNF-α, TNF-β, VEGF, Eotaxin/CCL11
323-plexHuman Cytokine/Chemokine Panel III6Ckine, BCA-1, CTACK, ENA-78, Eotaxin-2, Eotaxin-3, I-309, IL-16, IL-20, IL-21, IL-23, IL-28A, IL-33, LIF, MCP-2, MCP-4, MIP-1d, SCF, SDF-1A+β, TARC, TPO, TRAIL, TSLP
425-plexHuman Th17 PanelGM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α, TNFβ
55-plexHuman Diabetes PanelInsulin, Glucagon, Leptin, GLP-1 (Active), C-Peptide
611-plexHuman Kidney Injury PanelCalbindin, Collagen IV, FABP1, GSTα, GSTπ, IP-10, KIM-1, Osteoactivin, Renin, TFF-3, TIMP-1
78-plexHuman Src Family Kinase Panel – PhosphoproteinBlk (Tyr389), Fgr (Tyr412), Fyn (Tyr420), Hck (Tyr411), Lck (Tyr394), Lyn (Tyr397), Src (Tyr419), Yes (Tyr421)
817-plexHuman Angiogenesis/Growth Factor PanelAngiopoietin-2, BMP-9, EGF, Endoglin, Endothelin-1, FGF-1, FGF-2, Follistatin, G-CSF, HB-EGF, HGF, IL-8, Leptin, PLGF, VEGF-A, VEGF-C, VEGF-D
97-plexDNA Damage/Genotoxicity PanelATR (Total), Chk1 (Ser345), Chk2 (Thr68), H2A.X (Ser139), MDM2 (total), p21 (Total), p53 (Ser15)
106-plexHuman Apolipoprotein PanelApo AI, Apo AII, Apo B, Apo CII, Apo CIII, Apo E
1112-plexHuman Bone Metabolism PanelACTH, DKK-1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin, OPN – Osteopontin, Osteoprotegerin, PTH, SOST, TNF-α
129-plexHuman Cancer/ Metastasis Biomarker PanelDKK-1, GDF-15, NSE, OPG, Osteonectin (SPARC), Periostin, TRAP5, TWEAK, YKL40
1310-plexHuman Cardiovascular Disease (CVD) Panel 1ADAMTS13, D-Dimer, GDF-15, Myoglobin, sICAM-1, MPO, P-selectin, Lipocalin-2/NGAL, sVCAM-1, SAA.
1414-plexHuman Cardiovascular Disease (Acute Phase) Panel 2AGP, Haptoglobin, SAP, Adipsin,  Human a2, CRP, Fetuin, Fibrinogen, L-Selectin, PF4, vonWillebrand Factor
1524-plexHuman Circulating Cancer Biomarker Panel IAFP, CEA, CYFRA21-1, FGF-2, HE4, PSA Total, PSA free, HGF, IL-6, IL-8, Leptin, MIF, OPN – Osteopontin, Prolactin, SCF, sFas, sFasL, TGF-α, TNF-α, TRAIL, VEGF, CA125, CA15-3, CA19-9, β-HCG
165-plexHuman Circulating Cancer Biomarker Panel 2Antithrombin III, Complement Factor H, Extracellular Matrix Protein 1, Vitamin D Binding Protein, Vitronectin
177-plexHuman Early Apoptosis PanelAkt (Ser473), BAD (Ser112), Bcl-2 (Ser70), Active Caspase 8 (Asp384), Active Caspase 9 (Asp315), JNK (Thr183/Tyr185), p53 (Ser46)
1811-plexHuman Kidney Injury Panel 1Calbindin, Collagen IV, FABP1, GSTα, GSTπ, IP-10, KIM-1, Osteoactivin, Renin, TFF-3, TIMP-1
197-plexHuman Kidney Injury Panel 2α-1-Microglobulin, Albumin, Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, Osteopontin (OPN)
2113-plexHuman Metabolic Hormone PanelAmylin (Total), Amylin (Active), C-Peptide, Ghrelin, GIP, GLP-1 (Active), GLP-1 (Total), Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, TNF-α
209-plexHuman Liver Protein Panel – MetabolismAFP, ANGPTL3, ANGPTL4, ANGPTL6/AGF, FABP1, FGF-19, FGF-21, FGF-23, HGF
2113-plexHuman Metabolic Hormone PanelAmylin (Total), Amylin (Active), C-Peptide, Ghrelin, GIP, GLP-1 (Active), GLP-1 (Total), Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, TNF-α
2214-plexHuman Soluble Cytokine Receptor PanelsCD30, sEGFR, sgp130, sIL-1RI, sIL-1RII, sIL-2Rα, sIL-4R, sIL-6R, sRAGE, sTNFRI, sTNFRII, sVEGF-R1, sVEGF-R2, sVEGF-R3
237-plexT-Cell Receptor Signaling PanelCD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr)
245-plexHuman Neurodegenerative Disease PanelHuman Neurodegenerative Disease Panel
253-plexHuman Sepsis PanelFGF-13, Olfactomedin-4 (OLFM-4), MMP-8
267-plexHuman Skin Immune Response PanelCortisol, HSA, Fibronectin, LPS, Involucrin, Keratin-6, Keratin-1,10
2713-plexHuman High Sensitivity T Cell PanelGM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, TNF-α
287-plexTotal Mitogenesis RTK PanelEGFR (Total), ErbB2/HER2 (Total), ErbB3/HER3 (Total), ErbB4/HER4 (Total), IR (total), c-Met/HGFR (Total), IGF-1R (Total)
299-plexMulti-Pathway Total Cell Signaling PanelErk/MAPK 1/2 (Total), Akt (Total), STAT3 (Total), JNK (Total), p70 S6 Kinase (Total), NFκB (Total), STAT5A/B (Total), CREB (Total), p38 (Total)
305-plexHuman Oxidative Stress Magnetic Bead Panel – Cellular Metabolism Multiplex AssayCatalase, PRX2, SOD1, SOD2, TRX1

ELISA

ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones.

Serum samples from 4 patients were collected three days (pre, Day1 and Day7) and at two time points for Day 1 and Day7. The IL-17A levels in these samples were analysed using a commercially available ELISA kit.

Request a consultation with Cellomatics Biosciences today

Our experienced team of in vitro laboratory scientists will work with you to understand your cell-based assay development needs and provide a bespoke project plan with a professional, flexible service and a fast turnaround time.

To request a consultation where we can discuss your exact requirements, please contact Cellomatics Biosciences.