Phagocytosis is a process mediated by a specialised group of innate immune cells called phagocytes, including neutrophils, monocytes and macrophages. Phagocytosis is an essential process to maintain tissue homeostasis.
Monocytes (CD14+) were isolated from PBMCs of healthy donors followed by treatment with M-CSF for 6 days. On day 6, the naïve macrophages were exposed to GM-CSF + IFNγ and M-CSF to generate M1 and M2 macrophages (MΦ) respectively. The cells were then exposed to fluorescent zymosan particles (green) and incubated for 3 hours. After 3 hours, the cells were washed, stained with fluorescent membrane marker (red) and DAPI (blue) for nucleus.
The images above showed M2 macrophages engulfed higher numbers of zymosan particles when compared to M1 macrophages (Objective= 20X).
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