Histology and IHC
This is an imaging technique to aid microscopic anatomy of biological tissues.
Normal Human Bronchial Epithelial Cells were grown on Air Liquid Interface (ALI) and allowed to differentiate for 28 days. At the end of the culture the cells were fixed and embedded into paraffin. The paraffin blocks were sectioned at 5µm thickness using Microtome and fixed onto slides. Haematoxylin and Eosin (H & E) staining were performed and slides were imaged at 4, 20 and 40X magnification. Distinct goblet and ciliated cells were observed on Day 28 of the ALI differentiation
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