ADCP: Antibody Dependent Cellular Phagocytosis
ADCP is a mechanism by which macrophages engulf and eliminate the tumour cells targeted with monoclonal antibodies.
The target Raji cells were pre-treated with or without Rituximab and stained with pHrodo Red Cell Labelling (red). The THP-1 derived macrophages were stained with calcein staining (green). To assess antibody-dependent cellular phagocytosis of target Raji cells by THP-1 derived macrophages the two cell types were co-cultured for 6 hours. Data is represented as the red fluorescence intensity (RFI) at 0 hours vs. 6 hours in all treatment groups.
Prior to initiating the co-culture with THP-1 derived macrophages, Raji cells were treated with Rituximab (RTX) for 15 minutes. Staining of Raji cells with Incucyte® phRodo® dye produces red fluorescent signals as a result of phagocytosis in response to the treatment conditions. Data is represented as Red Fluorescence Intensity that is a direct measure of phagocytosis.
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