Western blotting is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or cell extracts.
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Western blotting – near IR imaging
Human Primary Epithelial Cells were transfected with the following ON TARGETplus smart pool reagents: non-targeting control (siCTRL) and siACTB using Lipofectamine.
A] Near IR imaging of Human Primary Epithelial Cell lysates captured 24 hours post-transfection, after SDS-PAGE separation and immunoblotting using anti-β-actin and anti-GAPDH antibodies.
B] 3D densitometry calculated using the Q9 Alliance© Software, for figures reported in A]. Analysis confirmed reduction of β-actin band in cells transfected with the siACTB, when compared to controls (rVI=relative volume intensity).
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