Allergic rhinitis is an inflammatory disorder of the nasal mucosa induced by allergen exposure triggering IgE-mediated inflammation. It can also be associated with co-morbid conditions as Asthma, Atopic Dermatitis & Nasal polyps (Varshney et al., 2015; doi: 10.1007/s12070-015-0828-5).
Nasal Epithelial cells and PBMC Co-culture Allergic Rhinitis model: Inflammatory mediator expression
Figure 1. Effect of Cetirizine and Chlorpheniramine maleate on pro-inflammatory markers. RPMI 2650 cells differentiated for 21 days and freshly isolated PBMC cells were grown in co-culture. They were then stimulated with House Dust Mite Extract (HDME) to induce an allergic rhinitis phenotype in the presence or absence of a low and high concentration of the drugs. The levels of TNF-α, IL-1α, IL-1β, IL-6, and IL-8 released in the supernatants were determined using multiplex immunoassay technique. One-way ANOVA was performed to determine statistical significance (n=3±SEM; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). Note: Unstimulated = RPMI 2650 + PBMCs, Stimulated = RPMI 2650 + PBMCs + HDME.
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