Allergic Rhinitis
Allergic rhinitis is an inflammatory disorder of the nasal mucosa induced by allergen exposure triggering IgE-mediated inflammation. It can also be associated with co-morbid conditions as Asthma, Atopic Dermatitis & Nasal polyps (Varshney et al., 2015).
Caco2 and PBMC Co-culture Allergic Rhinitis model: TEER Measurements
RPMI 2650 cells differentiated for 21 days and freshly isolated PBMC cells were grown in co-culture and stimulated with House Dust Mite Extract (HDME) to induce an allergic rhinitis phenotype in the presence or absence of Cetirizine and Chlorphenamine maleate. The TEER reported for all the conditions (compounds and time of treatment) were normalized to the TEER reported on day 21 of differentiation (Basal) for each of the transwells. This data was analyzed for both compounds using Two-way ANOVA followed by Dunnett’s multiple comparisons test [95% CI of diff.] (*p<0.05, **p<0.01,****p<0.0001). For each time point, all the conditions are compared to the Stimulated control.
Note: Unstimulated = RPMI 2650 + PBMCs, Stimulated= RPMI 2650 + PBMCs + HDME
Caco2 and PBMC Co-culture Allergic Rhinitis model: Inflammatory mediator expression
Significantly increased levels of pro-inflammatory markers were observed after stimulation of the differentiated RPMI 2650 monolayers with House Dust Mite Extract (HDME) when compared to the unstimulated control. The levels of these markers were suppressed when the RPMI 2650 monolayers were pre-treated with both reference compounds. Data was analyzed using Ordinary One-way ANOVA followed by Dunnett’s multiple comparisons test [95% CI of diff.] (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns= not significant). All the conditions were compared to the stimulated control for each of the markers and showed significant differences.
Note: Unstimulated = RPMI 2650 + PBMCs, Stimulated= RPMI 2650 + PBMCs + HDME
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