Invasion Assays

Cell invasion assays are finely regulated processes that are critical in many normal physiological processes including during embryonic development, wound repair and immune surveillance (Lauffenburger and Horwitz, 1996; Pollard and Borisy, 2003; Ridley et al., 2003). However, these dynamic cell movements are also crucial in cancer progression and metastasis (Hamidi and Ivaska, 2018).

Here at Cellomatics, we support a wide range of projects that involve a focus on invasion assays.

Migration & Invasion

Migration: HT-1080 and 3T3-LI into Corning Transwell™ inserts (Pore size: 0.8 μm) in serum-free media and allowed to migrate towards FBS for 48hrs in the presence or absence of  Cytochalsain D. Migration assay showed that treatment with Cytochalsain D resulted in a significant decrease in migration with highly invasive HT-1080 cells, as indicated in the relative fluorescent values.

Invasion: HT-1080 and 3T3-LI were seeded into Corning Transwell™ inserts (Pore size: 0.8 μm) and were coated with Cultrex® Basement Membrane Extract. Cells seeded in serum-free media invaded towards FBS for 48hrs in the presence or absence of Cytochalsain D. Invasion assay showed that treatment with 2µM Cytochalsain D resulted in a significant decrease in invasion with highly invasive HT-1080 cells, as indicated in the relative fluorescent values.

Request a consultation with Cellomatics Biosciences today

Our experienced team of in vitro laboratory scientists will work with you to understand your project and provide a bespoke project plan with a professional, flexible service and a fast turnaround time.

To request a consultation where we can discuss your exact requirements, please contact Cellomatics Biosciences.