Cell invasion assays are finely regulated processes that are critical in many normal physiological processes including during embryonic development, wound repair and immune surveillance (Lauffenburger and Horwitz, 1996; Pollard and Borisy, 2003; Ridley et al., 2003). However, these dynamic cell movements are also crucial in cancer progression and metastasis (Hamidi and Ivaska, 2018).
Here at Cellomatics, we support a wide range of projects that involve a focus on invasion assays.
Migration & Invasion
Migration: HT-1080 and 3T3-LI into Corning Transwell™ inserts (Pore size: 0.8 μm) in serum-free media and allowed to migrate towards FBS for 48hrs in the presence or absence of Cytochalsain D. Migration assay showed that treatment with Cytochalsain D resulted in a significant decrease in migration with highly invasive HT-1080 cells, as indicated in the relative fluorescent values.
Invasion: HT-1080 and 3T3-LI were seeded into Corning Transwell™ inserts (Pore size: 0.8 μm) and were coated with Cultrex® Basement Membrane Extract. Cells seeded in serum-free media invaded towards FBS for 48hrs in the presence or absence of Cytochalsain D. Invasion assay showed that treatment with 2µM Cytochalsain D resulted in a significant decrease in invasion with highly invasive HT-1080 cells, as indicated in the relative fluorescent values.
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