Case Study: Permeability assays- analysis of calcium transport across cell monolayers
Summary An experimental model was optimised to assess functional modification of the permeability of cellular monolayers to calcium. Cells were cultured on transwell inserts in a multi-well plate format. Once monolayers had reached confluency, electrical resistance measurements (TEER) were used to determine barrier function. Apical chambers were subsequently incubated with test compounds and calcium. Barrier […]
Case Study: Target Validation part two
Initial client request “To support the positive therapeutic evidence obtained through the existing cell-based screening assay at Cellomatics we are looking for a supplementary method to validate the effects seen for certain drug candidates. This must confirm target engagement is specific for the protein target and the effects observed are directly related to this engagement.” […]
Case Study: Target Validation
Initial client request “Looking to validate a gene target identified via DepMap for potential druggability across a range of cancer types. The end goal being to test a compound series designed with an advance computational genomics platform against said target” Proposed experimental approach Number of cell lines: 8 o2 x cell lines (1 predicted sensitive […]