INNOBEX™ in vitro Blood Brain Barrier (BBB) Model

The blood brain barrier (BBB) presents one of the greatest challenges in the development of novel CNS therapeutics . Responsible for the transport of essential nutrients into the brain while simultaniously protecting from hazardous substances or pathogens in the bloodstream, the BBB is a highly complex and selective stucture. Here at Cellomatics, Our trademark model INNOBEX™ consists of primary human brain microvascular endothelial cells (HBMVECs), astrocytes, and pericytes in a 3D transwell format to simulate the separation between the blood (apical chamber) and the brain (basal chamber) whilst maintaining cell-to-cell interactions and forming stable barrier properties. Our INNOBEX™ model has been validated for the expression of tight junction proteins, common transporter targets, and by using trans-endothelial electrical resistance (TEER) and FITC-Dextran we have demonstrated that barrier integrity can be manipulated in response to different stimuli.