macrophage polarisation
Immunology Models
Macrophage Polarisation Assay
Due to their plasticity, macrophages orchestrate complex, differential cellular actions to specific microenvironmental cues, via the innate immune response. Cellomatics offers both M1 and M2 macrophage polarisation assays starting from freshly isolated monocytes. Studies can accommodate individual or pooled donor analysis. Immunomodulatory effects of test therapeutics can be determined by a combination of cytokine release profiling and cell surface marker characterisation.
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Available formats
Standard 96-well format.
FAQ's
Macrophages exhibit functional plasticity, shifting between pro-inflammatory (M1) and anti-inflammatory (M2) states. This balance plays a critical role in diseases such as cancer, where M2-like macrophages can suppress immune responses, and inflammatory disorders, where M1 activity contributes to tissue damage.
Primary human monocytes are differentiated into macrophages and stimulated under defined conditions to induce M1 or M2 phenotypes. Phenotypic and functional changes are then assessed using flow cytometry, gene expression analysis, or multiplex cytokine profiling. This process is supported at Cellomatics through in-house isolation of primary cells and optimised culture conditions, ensuring high-quality, physiologically relevant data.
Yes. These assays determine whether a compound promotes, inhibits, or reprogrammes macrophage polarisation, which is particularly relevant for immuno-oncology and inflammation research. Cellomatics integrates these studies with multi-parametric readouts and disease-relevant models to provide robust insights into compound mechanism and immune modulation.
Key readouts include cytokine secretion, surface marker expression, and functional activity, providing a comprehensive assessment of macrophage phenotype. At Cellomatics, these outputs are generated using advanced analytical platforms and primary human cells to ensure reproducibility and translational relevance.
Cellomatics leverages primary human cells and advanced analytical platforms to deliver physiologically relevant and reproducible data, supported by customised study design.