Immunology Models
phagocytosis Assay
Phagocytosis Assay
Phagocytosis is a physiological process mediated by a specialised group of innate immune cells that engulf and digest microorganisms, foreign substances or apoptosing cells. These cells, phagocytes, include neutrophils, monocytes and macrophages and their actions are essential to maintaining tissue homeostasis. Our expertise in immune cell isolation and handling allows us to offer highly customisable and physiologically relevant assays designed to best suit your research interests in phagocytic cell activity.
Available formats
Standard 96-well format.
Alternative format offerings available upon request.
Pilot assay development is available as a standalone service, or as a built in checkpoint for full studies
FAQ's
A phagocytosis assay measures the ability of immune cells, such as macrophages or neutrophils, to recognise, internalise, and degrade target particles or cells. It provides a functional readout of innate immune activity.
Phagocytosis is influenced by factors such as target opsonisation, receptor engagement, and effector cell type. Antibody coating can enhance Fc receptor-mediated uptake, while assay conditions such as incubation time and cell ratios affect signal strength.
They provide functional validation of antibody-mediated clearance mechanisms, particularly ADCP. This goes beyond binding affinity and helps rank candidates based on biological activity. Cellomatics incorporates phagocytosis assays into antibody characterisation workflows.
Sensitivity depends on labelling strategies, detection methods, and target design. Ensuring that signal reflects true internalisation rather than surface binding is critical for accurate interpretation.
Phagocytosis assays are typically performed using flow cytometry or high-content imaging. These platforms allow quantification at both population and single-cell levels. Cellomatics offers both approaches depending on study requirements.
Distinguishing internalised targets from surface-bound material is essential. Techniques such as fluorescence quenching and confocal imaging are used to confirm intracellular localisation, ensuring data accuracy.